Milk fat globule epidermal growth factor 8 alleviates liver injury in severe acute pancreatitis by restoring autophagy flux and inhibiting ferroptosis in hepatocytes

Figure 2 Milk fat globule epidermal growth factor 8 deficiency aggravated hepatic injury and inflammation in experimental severe acute pancreatitis. In mice, acute pancreatitis (AP) was induced by 2 hly intraperitoneal injections of 4.0 g/kg L-arginine. The animals were sacrificed at 72 h after the first injection of L-arginine. Blood and tissue samples were collected. A: Representative photos of hematoxylin and eosin staining in the liver (400 × or 1000 ×); B: Hepatic injury scores; C: Percentages of necrotic areas; D: Tumor necrosis factor-α levels in the liver; E: Myeloperoxidase levels in the liver; F and G: Representative photos of TdT-mediated dUTP Nick-End Labeling (TUNEL) staining (400 ×) and quantitative of TUNEL staining. n = 3-6/group, error bars indicate the SEM; ^aP < 0.05 vs sham group; ^bP < 0.05 vs vehicle group. SAP: Severe acute pancreatitis; KO: Knockout; WT: Wild type; TNF-α: Tumor necrosis factor-α; MPO: Myeloperoxidase; TUNEL: TdT-mediated dUTP Nick-End Labeling.