Recombinant adeno-associated virus 8-mediated inhibition of microRNA let-7a ameliorates sclerosing cholangitis in a clinically relevant mouse model

Figure 6 Recombinant adeno-associated virus 8 mediated let-7a-5p inhibitor interfers suppressor of cytokine signaling 1/Dectin1 mediated NF-κB signaling pathway. The expression of suppressor of cytokine signaling 1 (SOCS1)/Dectin1/P-p65 in each mouse of normal control group, anti-scramble control and anti-let-7a-5p group were assayed by western-blot. A: The blot of SOCS1/Dectin1/ P-p65 in each group after 2 wk 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine (DDC) feeding; B-D: the relative levels of SOCS1, Dectin1, and P-p65 in each group after 2 wk DDC feeding; E: The blot of SOCS1/Dectin1/P-p65 in each group after 6 wk DDC feeding; F-H: relative levels of SOCS1, Dectin1, and P-p65 in each group after 6 wk DDC feeding. DDC: 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine; SCR: Scramble control. ^aP < 0.05, ^bP < 0.01.