Recombinant adeno-associated virus 8-mediated inhibition of microRNA let-7a ameliorates sclerosing cholangitis in a clinically relevant mouse model

Figure 5 Let-7a-5p inhibition mediated by recombinant adeno-associated virus 8 ameliorates biliary fibrosis in the 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine-induced cholestasis model. A: Sirius Red staining in the liver of mice at 2 wk and 6 wk after 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine feeding; B and C: The fibrosis markers such as Acta 2 and Tgfb were evaluated using qRT-PCR; D: The contents of Hyp in the liver of mice in each group were determined. DDC: 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine; SCR: Scramble control; Hyp: Hydroxyproline. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.