Recombinant adeno-associated virus 8-mediated inhibition of microRNA let-7a ameliorates sclerosing cholangitis in a clinically relevant mouse model

Figure 4 Let-7a-5p inhibition delivered by recombinant adeno-associated virus 8 prevents liver pathology and the proliferation of cholangiocytes. A: Histological changes at 2 and 6 wk was evaluated using hematoxylin and eosin staining. The upper panel was shown in 100 times amplification; the nether panel was shown in 200 times amplification; B: CK19 was stained for indicating the proliferation of cholangiocytes using Immunohistochemistry staining; C: and the percent of CK19 positive cells in the liver was calculated at 2 and 6 wk. DDC: 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine; SCR: Scramble control. ^cP < 0.001.