Recombinant adeno-associated virus 8-mediated inhibition of microRNA let-7a ameliorates sclerosing cholangitis in a clinically relevant mouse model

Figure 2 The expression of let-7a-5p is depressed using anti-let-7a-5p sponges delivered by recombinant adeno-associated virus 8. A: The recombinant adeno-associated virus 8 was extensively distributed in the liver of mice after 2 or 6 wk of 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine (DDC) feeding; B and C: The relative expression of GFP indicates the relative amounts of virus; D and E: The relative expression of let-7a-5p in the liver of mice injected by adeno-associated virus 8-mediated scramble control or anti-let-7a-5p sponges after 2 wk or 6 wk of DDC feeding. DDC: 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine; SCR: Scramble control. ^bP < 0.01, ^cP < 0.001.