Recombinant adeno-associated virus 8-mediated inhibition of microRNA let-7a ameliorates sclerosing cholangitis in a clinically relevant mouse model

Figure 1 Schematic representation of recombinant adeno-associated virus 8 delivered let-7a-5p inhibitor and the design of experiment. A: Diagrams of anti-let-7a-5p sponges’ vector and anti-scramble control (SCR) control vector; B: The design of the experiment. The mice were divided 3 groups (n = 6 for each group): (1) Normal control mice with feeding chow; (2) 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine (DDC) feeding mice who were iv injected with adeno-associated virus 8-anti-SCR at the dose of 4 × 10¹¹ vg per mouse; and (3) DDC feeding mice were iv injected with anti-let-7a-5p sponges (anti-let-7a-5p) at the dose of 4 × 10¹¹ vg per mice. The mice in each group were sacrificed at 2 wk and 6 wk after DDC feeding to evaluate the expression of let-7a-5p and the therapeutic effects of let-7a-5p inhibitor. ITR: Invert terminal repeat; TBG: Thyroxine-binding globulin; DDC: 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine; AAV: Adeno-associated virus.