N6-methyladenosine-modified long non-coding RNA KIF9-AS1 promotes stemness and sorafenib resistance in hepatocellular carcinoma by upregulating SHOX2 expression

Figure 5 The long noncoding RNA KIF9-AS1 enhances the stability and expression of short stature homeobox 2 through ubiquitin-specific peptidase 1-mediated short stature homeobox 2 deubiquitination. A: The ubiquitination levels of short stature homeobox 2 in Huh-7 and Huh-7/R cells were determined via immunoprecipitation and western blotting; B: Western blotting was conducted to analyze the short stature homeobox 2 protein. ^aP < 0.05, ^bP < 0.01. Each experiment was repeated three times. USP1: Ubiquitin-specific peptidase 1; SHOX2: Short stature homeobox 2; CHX: Cyclohexamide.