Establishment of a chronic biliary disease mouse model with cholecystoduodenal anastomosis for intestinal microbiome preservation

Figure 5 Quantitative reverse transcription-polymerase chain reaction analysis of 16s rRNA and ipaH genes in the ileum and bile of cholecystoduodenal anastomosis mice after injection of bacteria. A: The mixture of Escherichia coli and Enterococcus was orally administered to cholecystoduodenal anastomosis mice. Bacterial gene was detected in the ileum using specific 16s rRNA primers; B-F: Cycle threshold (CT) of bacterial gene expression detected in the ileum and bile using EIEC-specific ipaH gene primers and 16s rRNA primers. Values are normalized to GAPDH levels and are represented as the mean ± SD. ^aP < 0.01, ^bP < 0.001 vs corresponding controls. 2W: 2 weeks after injection; 4W: 4 weeks after injection; CTL: Control; E. coli: Escherichia coli.