Establishment of a chronic biliary disease mouse model with cholecystoduodenal anastomosis for intestinal microbiome preservation

Figure 1 Schematic of gallbladder incision and duodenal anastomosis. A and B: Incision site of skin and peritoneum of C57BL/6 mice; C: A suture for connecting the gallbladder and duodenum is positioned at 12 o'clock (also referred to as 0°), 5 o'clock (or 150°), and 9 o'clock (or 270°) in an interrupted manner.