Quantitative proteomics analysis reveals the pathogenesis of obstructed defecation syndrome caused by abnormal expression of dystrophin

Figure 5 Downregulation of dystrophin expression leads to dysregulation of calcium influx in human intestinal smooth muscle cells. A: Representative traces of L-type calcium currents (ICa, L) were recorded from human intestinal smooth muscle cells transfected with negative control small interfering RNA (siR-NC) or siR-dystrophin (siR-DMD) using patch-clamp techniques (the X-axis represents the time in milliseconds and the Y-axis represents the current magnitude in pico-amps); B: Line graph of ICa-L current density. The inward current induced by depolarization voltage increased after siR-DMD transfection; C: Peak intracellular calcium concentrations in the two groups of human intestinal smooth muscle cells; D: Average difference between cells transfected with siR-NC or siR-DMD shown in the estimated graph above. Both groups are plotted on the left Y-axis; the mean difference is shown as a bootstrap sampling distribution on the floating axis on the right. ^dP < 0.0001, n = 6. siR-NC: Negative control small interfering RNA; siR-DMD: Dystrophin small interfering RNA; DMD: Dystrophin; ICa-L: L-type calcium currents.