Quantitative proteomics analysis reveals the pathogenesis of obstructed defecation syndrome caused by abnormal expression of dystrophin

Figure 3 Downregulation of dystrophin expression is associated with impaired contraction of human intestinal smooth muscle cells. A: Assessment of contractile function in intestinal smooth muscle cells after transfection with negative control small interfering RNA (siR-NC) (top) or siR-dystrophin (siR-DMD) (bottom); B: Quantitative analysis of cell area after contraction in both groups; C: Detection of DMD expression in human intestinal smooth muscle cells transfected with siR-NC or siR-DMD by real-time quantitative polymerase chain reaction; D: Western blot detection of DMD expression in whole cell extracts of human intestinal smooth muscle cells transfected with siR-NC or siR-DMD; E: Quantitative analysis of Western blot greyscale value. ^bP < 0.01, ^dP < 0.0001, n = 3. siR-NC: Negative control small interfering RNA; siR-DMD: Dystrophin small interfering RNA; DMD: Dystrophin.