Quantitative proteomics analysis reveals the pathogenesis of obstructed defecation syndrome caused by abnormal expression of dystrophin

Figure 2 Downregulation of dystrophin in intestinal smooth muscle cells of obstructed defecation syndrome patients. A: Representative immunohistochemistry staining for dystrophin (DMD) in 50 rectal specimens from both groups. Scale bar: 100 μm (left) and 50 μm (right); B: Quantitative analysis of DMD immunohistochemistry expression [integrated optical density (IOD)] in rectal samples from the obstructed defecation syndrome (ODS) group compared with the control group; C: Correlation analysis between Wexner constipation score and IOD in ODS patients. Spearman correlation r = 0.7720, P < 0.0001; D: Correlation analysis between Wexner constipation score and Oxford score in ODS patients with rectal prolapse. Spearman correlation r = 0.0904, P > 0.1; E: Correlation analysis between Wexner constipation score and IOD in ODS patients. Spearman’s correlation r = 0.1348, P > 0.1; F: Representative immunofluorescence staining for DMD in 50 rectal specimens from both groups. Scale bar = 20 μm. Nuclei were counterstained with DAPI; G: Quantitative analysis of mean fluorescence intensity (MFI) relative to the ODS and control groups; H: Correlation analysis between MFI and Wexner constipation score in the ODS group. Spearman correlation r = 0.7025, P < 0.0001; I: Correlation analysis between MFI and Oxford grade in ODS patients with rectal prolapse. Spearman correlation r = 0.03458, P > 0.1. ^dP < 0.0001. ODS: Obstructed defecation syndrome; IOD: Integrated optical density; DMD: Dystrophin.