Amino acid deletions at positions 893 and 894 of cytotoxin-associated gene A protein affect Helicobacter pylori gastric epithelial cell interactions

Figure 5 Quantification of the F-actin cytoskeleton with FITC phalloidin stain. A: The average fluorescence intensity of F-actin at 12 hours and 24 hours after transfection. The data were mean ± SD of three independent experiments. Errors bars represented standard errors of the means. ^aP < 0.05, ^bP < 0.01; B: Representative micrographs of F-actin cytoskeleton as indicated (400 ×). AGS cells were labeled for F-actin using FITC phalloidin and the nucleus was stained with DAPI. F-actin was shown in green, and the nucleus was labeled with blue. 1 and 2 represented the F-actin cytoskeleton at 12 hours and 24 hours after transfection, respectively. Scale bar = 25 μm.