Amino acid deletions at positions 893 and 894 of cytotoxin-associated gene A protein affect Helicobacter pylori gastric epithelial cell interactions

Figure 1 Identification of cytotoxin-associated gene A and its mutated genes. A: The cytotoxin-associated gene A (cagA) gene was amplified by polymerase chain reaction (PCR) from the HZT strain. Lane M: DNA marker; lane 1 cagA gene; lane 2 Control; B: The mutated gene was amplified by splicing by overlap extension (SOE) PCR. Lane M: DNA marker; lane 1 cagA-NT gene; lane 2 cagA-NE gene; lane 3 Control; C: Sanger sequencing results; D: The target genes were amplified by PCR from eukaryotic expression recombinant plasmids. Lane M: DL8000 DNA marker; lane 1 pAdtrack/cagA; lane 2 pAdtrack/cagA-NT; lane 3 pAdtrack/cagA-NE; E: Double enzyme identification results. Lane M: DL8000 DNA marker; lane 1 pAdtrack/cagA; lane 2 pAdtrack/cagA-NT; lane 3 pAdtrack/cagA-NE. cagA: Cytotoxin-associated gene A.