Amlodipine inhibits the proliferation and migration of esophageal carcinoma cells through the induction of endoplasmic reticulum stress

Figure 6 Inhibition of autophagy enhanced amlodipine-induced apoptosis and migration. A and B: To investigate the effects of amlodipine, 3-methyladenine (3-MA), or their combination on cell proliferation and migration of KYSE-450 and Eca109 cells, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT) and Transwell migration assay were carried out, and significant results were observed with ^aP < 0.05 vs Control; C: LC3B, as well as E-cadherin content and levels, were tested in detail by means of western blot analysis, cleaved caspase 3 in KYSE-450 and Eca109 cells after treatment with amlodipine (4 μg/mL), 3-MA (3 mmol/L), or their combination. GAPDH served as a loading control. 3-MA: 3-methyladenine.