Fanlian Huazhuo Formula alleviates high-fat diet-induced non-alcoholic fatty liver disease by modulating autophagy and lipid synthesis signaling pathway

Figure 5 Fanlian Huazhuo Formula group regulated lipid synthesis signaling pathway in vitro. A: Protein expression levels of AMPKα, SREBP-1C, ACC1, FASN and β-actin in HepG2 cells induced by free fatty acid (n = 3); B: Relative mRNA expression levels of AMPKα, SREBP-1C, ACC1 and FASN in HepG2 cells tested by real-time quantitative polymerase chain reaction (n = 3). Data are presented as mean ± SE. ^aP < 0.05 vs NC group, ^cP < 0.05 vs MOD group. NC: Negative control group; MOD: Model group; FLHZF: Fanlian Huazhuo Formula group.