Fanlian Huazhuo Formula alleviates high-fat diet-induced non-alcoholic fatty liver disease by modulating autophagy and lipid synthesis signaling pathway

Figure 3 Effects of Fanlian Huazhuo Formula group on abnormal lipid levels and liver injuries in high-fat diet-induced mice. A: Serum levels of triglyceride in each group (n = 8); B: Serum levels of total cholesterol in each group (n = 8); C: Serum levels of low-density lipoprotein cholesterol in each group (n = 8); D: Serum levels of high-density lipoprotein cholesterol in each group (n = 8); E and F: Liver injury markers of aspartate aminotransferase and alanine aminotransferase in each group (n = 8); G: Representative images of livers with hematoxylin and eosin (HE) staining in each group, magnification 200 ×; H: Representative images of livers with Oil Red O staining in each group, magnification 400 ×; I: Nonalcoholic fatty liver disease Activity Score of HE staining (n = 3); J: Percentage of area positive for Oil Red O staining in liver tissue (n = 3). Data are presented as mean ± SE. ^aP < 0.05 vs NC group, ^cP < 0.05 vs MOD group, ^dP < 0.01 vs MOD group. TG: Triglyceride; TC: Total cholesterol; LDL-C: Low-density lipoprotein cholesterol; HDL-C: High-density lipoprotein cholesterol; AST: Aspartate aminotransferase; ALT: Alanine aminotransferase; NC: Negative control group; NAFLD: Nonalcoholic fatty liver disease; MOD: Model group; FNBT: Fenofibrate group; FLHZF-L: Low dose of Fanlian Huazhuo Formula group; FLHZF-H: High dose of Fanlian Huazhuo Formula group.