Fanlian Huazhuo Formula alleviates high-fat diet-induced non-alcoholic fatty liver disease by modulating autophagy and lipid synthesis signaling pathway

Figure 1 Fanlian Huazhuo Formula group reduced lipid droplets deposition in HepG2 cells induced by free fatty acid. A: Cell viability with Fanlian Huazhuo Formula group (FLHZF) treatment in free fatty acids (FFAs)-induced HepG2 cells tested by cell counting kit-8 (n = 3); B: Cell triglyceride (TG) content without or with FLHZF treatment in HepG2 cells (n = 3); C: Cell TG content with FLHZF treatment in FFA-induced HepG2 cells (n = 3); D: Percentage of area positive for Oil Red O staining in HepG2 cells (n = 3); E: Representative images of FFA-induced HepG2 cells of Oil Red O staining, magnification 400 ×. Data are presented as mean ± SE. ^aP < 0.05 vs NC group, ^cP < 0.05 vs MOD group. NC: Negative control group; SOL: Solvent group MOD: Model group; FLHZF: Fanlian Huazhuo Formula group; TG: Triglyceride.