Calculus bovis inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer

Figure 8 In vivo experimental validation of regulation of M2 macrophage polarization by Calculus bovis through the Wnt pathway. A: Quantification of mRNA expression of Wnt5B, β-catenin, and Axin2 by real-time reverse transcriptase-polymerase chain reaction; B: Quantification of protein expression of Wnt5B, β-catenin, and Axin2 by Western blot analysis; C: Ratio of cells expressing CD206 molecules in tumor tissues analyzed by flow cytometry. ^eP < 0.01.