Calculus bovis inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer

Figure 7 Calculus bovis-enriched serum inhibits polarization of M2 tumor-associated macrophages through the Wnt pathway to suppress against liver cancer. A: Effect of Calculus bovis-enriched serum (CBS) on Wnt5B, β-catenin, and Axin2 mRNA levels in THP-1 cells detected by real-time reverse transcriptase-polymerase chain reaction; B: Effect of CBS on Wnt5B, β-catenin, and Axin2 protein levels in THP-1 cells detected by Western blot analysis; C: Effect of the Wnt pathway agonist SKL2001 on β-catenin protein expression; D: Changes in CD206 detected by flow cytometry in THP-1 cells after CBS combined with SKL2001 intervention for polarization. ^eP < 0.01.