Calculus bovis inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer

Figure 6 Calculus bovis-enriched serum inhibits polarization of M2 tumor-associated macrophages through the Wnt pathway and suppresses malignant behaviors of liver cancer cells. A: Inhibition rate of HepG2 proliferation by M2 + Calculus bovis-enriched serum (CBS) conditioned medium; B: Effect of M2 + CBS conditioned medium on HepG2 apoptosis detected by flow cytometry; C: Inhibitory effect of M2 + CBS conditioned medium on migration of HepG2 cells by cell scratch assay; D: Inhibitory effect of M2 + CBS conditioned medium on migration of HepG2 cells by Transwell assay. M2 + CBS conditioned medium: Supernatants of differentiated M2 macrophages cultured in DMEM medium enriched with CBS and penicillin-streptomycin, and the conditional media were collected after culture. ^dP < 0.05, ^eP < 0.01.