Calculus bovis inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer

Figure 5 Calculus bovis-enriched serum inhibits polarization of M2 tumor-associated macrophages. A: Cytotoxicity of Calculus bovis-enriched serum (CBS) on THP-1 cells by Cell Counting Kit-8 assay. The blank group served as a control; B: CD206 quantification in THP-1 cells after IL-4 and IL-13 induction by flow cytometry; C: The mRNA expression levels of CD206 after intervention of M2 tumor-associated macrophages (M2-TAMs) with different concentrations of blank serum and CBS; D: Quantification of CD206 in M2-TAMs after 10% (100 mL/L) CBS intervention by flow cytometry; E: The mRNA expression levels of CCL22, TGF-β2, IL-10, and Arg-1 after 10% (100 mL/L) CBS intervention in M2-TAMs. ^dP < 0.05, ^eP < 0.01. BS: Blank serum; CBS: CB-enriched serum.