Loss of monopolar spindle-binding protein 3B expression promotes colorectal cancer invasiveness by activation of target of rapamycin kinase/autophagy signaling

Figure 7 Monopolar spindle-binding protein 3B inhibition of colorectal cancer cell malignant behaviors through inactivation of mechanistic target of rapamycin kinase/autophagy signaling. A: Western blot images. Stable monopolar spindle-binding protein 3B (MOB3B)-overexpressing RKO cells were grown, treated with MHY 1485 (50 μM) for 48 h, and then subjected to Western blot analysis; B: Quantified data of A; C-H: RKO cells with with stable MOB3B knockdown were grown, treated with rapamycin (100 nM) for 48 h, and then subjected to wound healing assay (C and D), Transwell assay (E and F), and Western blot analysis (G and H). The data are presented as the mean ± SD of three independent experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. mTOR: Mechanistic target of rapamycin; MMP: Matrix metalloproteinase; LC: Light chain.