Loss of monopolar spindle-binding protein 3B expression promotes colorectal cancer invasiveness by activation of target of rapamycin kinase/autophagy signaling

Figure 6 Effect of monopolar spindle-binding protein 3B on number of autophagosomes/autophagy lysosomes in RKO cells. A: Transmission electron microscopy (TEM) images. Stable monopolar spindle-binding protein 3B (MOB3B)-overexpressing RKO cells were grown and treated with MHY 1485 (50 μM), a mechanistic target of rapamycin kinase (mTOR) agonist, for 48 h, and then subjected to TEM analysis of cell morphology; B: Quantified data of A; C: TEM images. RKO cells with stable MOB3B knockdown were grown and treated with rapamycin (100 nM), an mTOR inhibitor, for 48 h, and then subjected to TEM analysis of cell morphology; D: Quantified data of C. The arrows show autophagosomes/autophagy lysosomes. The data are shown as the mean ± SD of five replicates. ^aP < 0.05 vs RKO-NC+, ^bP < 0.05 vs RKO-OE, ^cP < 0.05 vs RKO-NC-, ^dP < 0.05 vs RKO-KD.