Loss of monopolar spindle-binding protein 3B expression promotes colorectal cancer invasiveness by activation of target of rapamycin kinase/autophagy signaling

Figure 5 Effects of monopolar spindle-binding protein 3B on expression of matrix metalloproteinase 2, matrix metalloproteinase 9, phosphorylated mechanistic target of rapamycin kinase, and autophagy related proteins in RKO cells. A: Representative Western blot images. Stable monopolar spindle-binding protein 3B (MOB3B)-overexpressing RKO cells were grown and subjected to Western blot analysis; B: Quantified data of A; C: Representative Western blot images. RKO cells with stable MOB3B knockdown were grown and subjected to Western blot analysis; D: Quantified data of C. The data are summarized as the mean ± SD of three replicates. ^aP < 0.05, ^bP < 0.01. mTOR: Mechanistic target of rapamycin; MMP: Matrix metalloproteinase.