Loss of monopolar spindle-binding protein 3B expression promotes colorectal cancer invasiveness by activation of target of rapamycin kinase/autophagy signaling

Figure 2 Establishment of RKO and DLD1 cell subline with stable monopolar spindle-binding protein 3B overexpression or knockdown. A-D: Quantitative reverse transcription-polymerase chain reaction; E-H: Western blot; I: Quantified data of E; J: Quantified data of F; K: Quantified data of G; L: Quantified data of H. The data are summarized as the mean ± SD of three independent experiments. ^aP < 0.0001 vs RKO-NC+, ^bP < 0.0001 vs RKO-NC-, ^cP < 0.001 vs DLD1-NC+, ^dP < 0.0001 vs DLD1-NC-, ^eP < 0.001 vs RKO-NC+, ^fP < 0.001 vs RKO-NC-, ^gP < 0.001 vs DLD1-NC+, ^hP < 0.01 vs DLD1-NC-.