Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 13  Further verification of the effects of Thymoquinone on the initial expression of hypoxia-inducible factor-1α protein. A: Western blot assay was performed to detect hypoxia-inducible factor-1α (HIF-1α) expression in PANC-1 cells treated with MG132 at 0 h, 0.5 h, 1 h, 2 h and 4 h under normoxia condition; B: Statistical analysis of HIF-1α protein expression in PANC-1 cells treated with MG132 at 0 h, 0.5 h, 1 h, 2 h and 4 h under normoxia condition; C: Western blot assay was performed to detect HIF-1α expression in PANC-1 cells treated with MG132 + Thymoquinone (TQ) at 0 h, 0.5 h, 1 h, 2 h and 4 h under normoxia condition; D: Statistical analysis of HIF-1α protein expression in PANC-1 cells treated with MG132+TQ at 0 h, 0.5 h, 1 h, 2 h and 4 h under normoxia condition. TQ: Thymoquinone; HIF-1α: Hypoxia-inducible factor-1α.