Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 12  Effects of Thymoquinone on interaction between hypoxia-inducible factor-1α and HSP90. A: Immunoprecipitation assay was performed to detect the hypoxia-inducible factor-1α (HIF-1α) and HSP90 immunoprecipitation of PANC-1 cells in MG132 group and MG132 + Thymoquinone (TQ) group under normoxia condition; B: Western blot assay was performed to detect HIF-1α expression of PANC-1 cells in normal control (NC) group, TQ group, geldanamycin (GA) group and TQ+GA group under hypoxia condition; C: Statistical analysis of HIF-1α protein expression of PANC-1 cells in NC group, TQ group, GA group and TQ+GA group under hypoxia condition. ^aP < 0.01. NC: Normal control; TQ: Thymoquinone; GA: Geldanamycin; HIF-1α: Hypoxia-inducible factor-1α.