Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 11  Effects of Thymoquinone on ubiquitination degradation of hypoxia-inducible factor-1α protein mediated by von Hippel Lindau under normoxia condition. A: Western blot analysis was performed to detect the expression of von Hippel Lindau (VHL) protein and hypoxia-inducible factor-1α (HIF-1α) protein in Lenti-VHL group, Lenti-VHL + Thymoquinone (TQ) group, VHL knockdown (sh-VHL) group and sh-VHL+TQ group under normoxia condition; B: Statistical analysis of VHL protein expression in the Lenti-VHL group, Lenti-VHL+TQ group, sh-VHL group and sh-VHL+TQ group under normoxia condition; C: Statistical analysis of HIF-1α expression in Lenti-VHL group, Lenti-VHL+TQ group, sh-VHL group and sh-VHL+TQ group under normoxia condition. ^aP < 0.05; ^bP < 0.01. TQ: Thymoquinone; VHL: von Hippel Lindau; sh-VHL: VHL knockdown; HIF-1α: Hypoxia-inducible factor-1α.