Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 10  Expression of von Hippel Lindau protein in von Hippel Lindau overexpression and von Hippel Lindau knockdown cell models under normoxia condition. A: Western blot assay was performed to detect von Hippel Lindau (VHL) protein expression in cell models of control group, overexpressed empty carrier (OE-NC) group and VHL overexpressed (OE-VHL) group under normoxia condition; B: Statistical analysis of VHL protein expression in cell models of control group, OE-NC group and OE-VHL group under normoxia condition; C: Western blot assay was performed to detect VHL protein expression in cell models of control group, knockdown empty vector (sh-NC) group and VHL knockdown (sh-VHL) group under normoxia condition; D: Statistical analysis of VHL protein expression in cell models of control group, sh-NC group and sh-VHL group under normoxia condition. ^aP < 0.05; ^bP < 0.01. VHL: von Hippel Lindau; OE-NC: Overexpressed empty carrier; OE-VHL: VHL overexpressed; sh-NC: Knockdown empty vector; sh-VHL: VHL knockdown.