Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 9 Effects of Thymoquinone on hydroxylation of hypoxia-inducible factor-1αprotein. A: Western blot assay was performed to detect hypoxia-inducible factor-1α (HIF-1α) and OH-HIF-1α expression of PANC-1 cells in normal control (NC) group and Thymoquinone (TQ) group under normoxia and hypoxia conditions; B: Statistical analysis of HIF-1α protein expression of PANC-1 cells in NC group and TQ group under normoxia and hypoxia conditions; C: Statistical analysis of OH-HIF-1α protein expression of PANC-1 cells in NC group and TQ group under normoxia and hypoxia conditions. ^aP < 0.01. NC: Normal control; TQ: Thymoquinone; HIF-1α: Hypoxia-inducible factor-1α.