Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 8 Effects of Thymoquinone on ubiquitination degradation of hypoxia-inducible factor-1α protein under normoxia condition. A: Western blot assay was performed to detect hypoxia-inducible factor-1α (HIF-1α) expression of PANC-1 cells in normal control (NC) group, MG132 group, Thymoquinone (TQ) group and MG132+TQ group under normoxia condition; B: Statistical analysis of HIF-1α protein expression of PANC-1 cells in NC group, MG132 group, TQ group and MG132+TQ group under normoxia condition. ^aP < 0.01. NC: Normal control; TQ: Thymoquinone; HIF-1α: Hypoxia-inducible factor-1α.