Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 7 Effects of Thymoquinone on PI3K/AKT/mTOR upstream pathway of hypoxia-inducible factor-1αprotein under hypoxia condition. A: Western blot assay was performed to detect the expression of p-PI3K-p85α, p-AKT, p-mTOR, p-S6K, p-4E-BP1 and eIF-4E of PANC-1 cells in normal control (NC) group and Thymoquinone (TQ) group under hypoxia condition; B: Statistical analysis of the expression of p-PI3K-p85α, p-AKT, p-mTOR, p-S6K, p-4E-BP1 and eIF-4E of PANC-1 cells in NC and TQ groups under hypoxia condition. ^aP < 0.05; ^bP < 0.01. NC: Normal control; TQ: Thymoquinone.