Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 6 Effects of Thymoquinone on hypoxia-inducible factor-1αmRNA and protein expression in PANC-1, AsPC-1 and BxPC-3 cells under hypoxia condition. A: Statistical analysis of hypoxia-inducible factor-1α (HIF-1α) mRNA expression of PANC-1, AsPC-1 and BxPC-3 cells in normal control (NC) group and Thymoquinone (TQ) group under hypoxia condition; B: Western blot assay was performed to detect HIF-1α protein expression of PANC-1, AsPC-1 and BxPC-3 cells in NC group and TQ group under hypoxia condition; C: Statistical analysis of HIF-1α protein expression of PANC-1, AsPC-1 and BxPC-3 cells in NC group and TQ group under hypoxia condition. ^aP < 0.01. NC: Normal control; TQ: Thymoquinone; HIF-1α: Hypoxia-inducible factor-1α.