Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Figure 1 Cell counting kit-8 assay was performed to determine the effects of different concentrations of Thymoquinone on the proliferation rate of pancreatic cancer cells and pancreatic duct epithelial cells under hypoxia condition. A: Cell counting kit-8 (CCK-8) assay was performed to detect the effects of Thymoquinone (TQ) on the relative proliferation rate of PANC-1 cells under hypoxia condition; B: CCK-8 assay was performed to detect the effects of TQ on the relative proliferation rate of AsPC-1 cells under hypoxia condition; C: CCK-8 assay was performed to detect the effects of TQ on the relative proliferation rate of BxPC-3 cells under hypoxia condition; D: CCK-8 assay was performed to detect the effects of TQ on the relative proliferation rate of normal pancreatic duct epithelial cells under hypoxia condition. TQ: Thymoquinone.