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©The Author(s) 2024.
World J Gastroenterol. Jun 7, 2024; 30(21): 2793-2816
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793
Table 8 Objective fragment double enzyme digestion process
Constituent | Dose |
1 × Tango Buffer | 2.0 μL |
BamH I | 0.5 μL |
EcoR I | 0.5 μL |
Objective fragment | 8.0 μL |
ddH2O | 9.0 μL |
Total dose | 20.0 μL |
- Citation: Zhao ZX, Li S, Liu LX. Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways. World J Gastroenterol 2024; 30(21): 2793-2816
- URL: https://www.wjgnet.com/1007-9327/full/v30/i21/2793.htm
- DOI: https://dx.doi.org/10.3748/wjg.v30.i21.2793