Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Table 7 Preparation of sodium dodecyl sulfate-polyacrylamide gel electrophoresis concentrated glue

Reagent	Concentration
H2O	2 mL	3 mL	4 mL	6 mL
30% acrylamide (29:1)	0.5 mL	0.75 mL	1 mL	1.5 mL
1M TRIS-HCl (pH 6.8)	0.5 mL	0.75 mL	1 mL	1.5 mL
10%SDS	40 μL	60 μL	80 μL	120 μL
Ammonium persulfate	30 μL	45 μL	60 μL	90 μL
TEMED	4 μL	6 μL	8 μL	12 μL
Bulk volume	3 mL	4.5 mL	6 mL	9 mL

SDS: Sodium dodecyl sulfate.