Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

Table 6 Preparation of sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation glue

Reagent	Separation glue concentration (%)
	8%	10%	12%	15%	18%	20%
H2O	4.63 mL	4 mL	3.3 mL	2.3 mL	1.3 mL	0.63 mL
30% acrylamide (29: 1)	2.67 mL	3.3 mL	4 mL	5 mL	6 mL	6.67 mL
1.5M Tris-HCl (pH 8.8)	2.5 mL	2.5 mL	2.5 mL	2.5 mL	2.5 mL	2.5 mL
10%SDS	0.1 mL	0.1 mL	0.1 mL	0.1 mL	0.1 mL	0.1 mL
Ammonium persulfate	0.1 mL	0.1 mL	0.1 mL	0.1 mL	0.1 mL	0.1 mL
TEMED	5 μL	5 μL	5 μL	5 μL	5 μL	5 μL
Bulk volume	10 mL	10 mL	10 mL	10 mL	10 mL	10 mL

SDS: Sodium dodecyl sulfate.