Copyright
©The Author(s) 2024.
World J Gastroenterol. Jun 7, 2024; 30(21): 2793-2816
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793
Item | Temperature | Time | Cycle-index |
Predegeneration | 95 ℃ | 10 min | 1 |
Denaturation | 95 ℃ | 15 s | 40 |
Annealing elongation | 60 ℃ | 60 s | 40 |
Melting curve acquisition | 95 ℃ | 15 s | 1 |
60 ℃ | 60 s | 1 | |
95 ℃ | 15 s | 1 |
- Citation: Zhao ZX, Li S, Liu LX. Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways. World J Gastroenterol 2024; 30(21): 2793-2816
- URL: https://www.wjgnet.com/1007-9327/full/v30/i21/2793.htm
- DOI: https://dx.doi.org/10.3748/wjg.v30.i21.2793