Basic Study
Copyright ©The Author(s) 2024.
World J Gastroenterol. Jun 7, 2024; 30(21): 2793-2816
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793
Table 4 Real-time fluorescence quantitative polymerase chain reaction system
Constituent
Dose
cDNA4 μL
Forward Primer (10 μM)0.4 μL
Reverse Primer (10 μM)0.4 μL
SYBR Green Master Mix10 μL
50 × ROX Reference Dye 20.4 μL
Taq Plus DNA Polymerase1 μL
RNase-free ddH2O25 μL