Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

doi:10.3748/wjg.v30.i21.2793

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 7, 2024; 30(21): 2793-2816
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793

Table 4 Real-time fluorescence quantitative polymerase chain reaction system

Constituent	Dose
cDNA	4 μL
Forward Primer (10 μM)	0.4 μL
Reverse Primer (10 μM)	0.4 μL
SYBR Green Master Mix	10 μL
50 × ROX Reference Dye 2	0.4 μL
Taq Plus DNA Polymerase	1 μL
RNase-free ddH₂O	25 μL

Citation: Zhao ZX, Li S, Liu LX. Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways. World J Gastroenterol 2024; 30(21): 2793-2816
URL: https://www.wjgnet.com/1007-9327/full/v30/i21/2793.htm
DOI: https://dx.doi.org/10.3748/wjg.v30.i21.2793