Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

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Jun 7, 2024 (publication date) through Jul 17, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 7, 2024; 30(21): 2793-2816
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793

Table 2 Preparation of reverse transcriptional response system

Constituent	Dose
5 × HiScript II Select qRT SuperMix II	4 μL
Reaction liquid from step 1	16 μL
Reverse transcriptase	1 μL

Incubate at 50 ℃ for 15 min; Incubate at 85 ℃ for 5 s; Incubate at 4 ℃ for 10 min.

Citation: Zhao ZX, Li S, Liu LX. Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways. World J Gastroenterol 2024; 30(21): 2793-2816
URL: https://www.wjgnet.com/1007-9327/full/v30/i21/2793.htm
DOI: https://dx.doi.org/10.3748/wjg.v30.i21.2793