Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

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Jun 7, 2024 (publication date) through Jul 17, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 7, 2024; 30(21): 2793-2816
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793

Table 14 Recombinant plasmid linking system

Constituent	Dose
10 × ligase Buffer	2.0 μL
pLVshRNA-EGFP(2A)Puro double enzyme digestion vector	2.0 μL
Target fragment	2.0 μL
Ligase	0.5 μL
ddH₂O	1.0 μL

Recombination at 22 ℃ for 2 h connection.

Citation: Zhao ZX, Li S, Liu LX. Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways. World J Gastroenterol 2024; 30(21): 2793-2816
URL: https://www.wjgnet.com/1007-9327/full/v30/i21/2793.htm
DOI: https://dx.doi.org/10.3748/wjg.v30.i21.2793