Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

doi:10.3748/wjg.v30.i21.2793

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Jun 7, 2024 (publication date) through Dec 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 7, 2024; 30(21): 2793-2816
Published online Jun 7, 2024. doi: 10.3748/wjg.v30.i21.2793

Table 11 Culture solution identified by polymerase chain reaction

Constituent	Dose
EntilinkTM PCR Master Mix	25.0 μL
Forward Primer (10 μM)	2.0 μL
Reverse Primer (10 μM)	2.0 μL
Template	Appropriate amount
ddH₂O	Up to 50 μL

37 ℃ water bath overnight. PCR: Polymerase chain reaction.

Citation: Zhao ZX, Li S, Liu LX. Thymoquinone affects hypoxia-inducible factor-1α expression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways. World J Gastroenterol 2024; 30(21): 2793-2816
URL: https://www.wjgnet.com/1007-9327/full/v30/i21/2793.htm
DOI: https://dx.doi.org/10.3748/wjg.v30.i21.2793