Hydrogen-rich water alleviates constipation by attenuating oxidative stress through the sirtuin1/nuclear factor-erythroid-2-related factor 2/heme oxygenase-1 signaling pathway

Figure 7 Expression of sirtuin1 in NCM460 cells is increased following treatment with β-leucine and traumatic acid, resulting in a reduction of H₂O₂-induced oxidative stress. A and B: CCK-8 assay of β-leucine (β-Leu) and traumatic acid in NCM460 cells; C and D: Reactive oxygen species (ROS) expression in response to β-Leu intervention; E and F: ROS expression in response to traumatic acid intervention; G: Sirtuin1 (SIRT1) expression following the administration of β-Leu and EX527; H: SIRT1 expression in response to the administration of traumatic acid and EX527. ^aP < 0.01 vs control group; ^bP < 0.01 vs H₂O₂; ^cP < 0.01 vs β-Leu; ^dP < 0.01 vs Traumatic acid. β-Leu: β-leucine; TA: Traumatic acid; ROS: Reactive oxygen species; Con: Control group.