Kinesin 26B modulates M2 polarization of macrophage by activating cancer-associated fibroblasts to aggravate gastric cancer occurrence and metastasis

Figure 6 KIF26B regulates M2 polarization of macrophages through fibroblast activation, thereby regulating invasiveness, angiogenesis, and epithelial-mesenchymal transition processes of gastric cancer cells. A: The migration ability of HGC-27 and AGS cells was examined by wound healing assay; B: The invasiveness of HGC-27 and AGS cells were evaluated by Transwell assay; C: Endothelial cell tube formation assay was applied to detect the ability of HGC-27 and AGS cells to form tubes; D: Western blotting assay was used to detect the expression levels of epithelial-mesenchymal transition related proteins; E: The number of M2-polarized macrophage infiltration in HGC-27 and AGS cells was counted by flow cytometry. n = 3. ^aP < 0.05, ^bP < 0.01. CAFs: Cancer-associated fibroblasts; sh-NC: sh-KIF26B negative controls.