Kinesin 26B modulates M2 polarization of macrophage by activating cancer-associated fibroblasts to aggravate gastric cancer occurrence and metastasis

Figure 5 KIF26B enhances crosstalk between cancer-associated fibroblasts and macrophages, thereby mediating M2 polarization of macrophages. A: Timer 2.0 analysis of the correlation between KIF26B expression and macrophage activation; B: Timer 2.0 analysis of the correlation between KIF26B expression and M2 type macrophages; C: The correlation between the expression of KIF26B and the level of CD163+ (M2 macrophage marker) infiltration was analyzed by immunohistochemistry. n = 3; D: Immunofluorescence detection of CD206 signaling in THP-1 cells. The supernatant of cancer-associated fibroblasts that transfected with sh-KIF26B was used to incubate THP-1 cells. n = 3; E: Flow cytometry was used to detect the number of M2-polarized macrophages. n = 3; F: Analyzing the correlation between KIF26B expression and chemokine CXCL12 expression through the Timer 2.0 database; G: Analyzing the correlation between KIF26B expression and CXCR4 expression trough the Timer 2.0 database. ^bP < 0.01. CAFs: Cancer-associated fibroblasts; sh-NC: sh-KIF26B negative controls.