Kinesin 26B modulates M2 polarization of macrophage by activating cancer-associated fibroblasts to aggravate gastric cancer occurrence and metastasis

Figure 4 KIF26B affects the activation of fibroblasts to form the cancer-associated fibroblast phenotype. A: Detection of transfection efficiency of sh-KIF26B negative controls/sh-KIF26B in HGC-27 cells by Western blotting; B: ELISA assay for detecting the expression of chemokines (CXCL12) in HFF cells; C: Detection of mRNA expression levels of CXCL12 in HFF cells by quantitative real-time polymerase chain reaction (qRT-PCR); D: Analyzing the invasive ability of HFF cells through Transwell assay; E: The expression levels of biomarkers (α-SMA, FAP, ITGB1, PDPN and THY1) for cancer-associated fibroblasts were detected by qRT-PCR. n = 3. ^aP < 0.05, ^bP < 0.01. sh-NC: sh-KIF26B negative controls.