Novel lactylation-related signature to predict prognosis for pancreatic adenocarcinoma

Figure 10  Inhibition of SLC16A1 expression reverses lactate-activated proliferation and migration. A: Expression of L-lactylation treated with C646; B: Colony formation assays of lactate, α-cyano-4-hydroxycinnamic acid (CHC), and C646 in PANC1 and SW1990 cells; C: Transwell assays of lactate, CHC, and C646 in PANC1 and SW1990 cells; D: Wound-healing assay of lactate, CHC, and C646 in PANC1 and SW1990 cells; E: Diagram of experimentation in vivo; F and G: Tumor formation in nude mice treated with CHC; H: Expression of L-lactyl in a xenograft of mice; I: Expression levels of L-lactyl in a xenograft of mice using immunofluorescence. Scale bar, 200 μm. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. CHC: Α-cyano-4-hydroxycinnamic acid; NC: Negative control.