Cell division cyclin 25C knockdown inhibits hepatocellular carcinoma development by inducing endoplasmic reticulum stress

Figure 4 Cell division cyclin 25C knockdown induced endoplasmic reticulum stress and activated endoplasmic reticulum stress-induced apoptosis in Hepa1-6 cells and the corresponding xenograft tumors. A and B: The expression of GRP78, XBP-1/s, CHOP, Caspase 9, and Caspase 3 in Hepa1-6 cells and the corresponding xenograft tumor tissues was analyzed at both the transcriptional and translational levels; C and D: The apoptosis rate of Hepa1-6 cells was assessed using flow cytometric analysis. The data represents the mean ± SD of three independent experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. CDC25C: Cell division cyclin 25C; GRP78: Glucose-regulated protein 78; XBP-1: X-box binding protein-1; XBP-1s: Splicing of X-box binding protein-1; CHOP: C/EBP homologous protein.