HepG2.2.15-derived exosomes facilitate the activation and fibrosis of hepatic stellate cells

Figure 1 Isolation of exosomes from HepG2. 2.15 cells. A: Analysis of the exosome marker protein CD9, expressed in HepG2.2.15, HepG2 and WRL68 cells, conducted via western blotting; B: Representative TEM examination images depicting exosomes secreted from HepG2.2.15 cells. Note scale bar: 0.1 nm; C: Determination of size distribution of the isolated exosomes, as measured by nanoparticle tracking analysis.